The Production of TNF-a by RAW 264.7 Macrophage Cells as a Result of Exposure to Aggregatibacter Actinomycetemcomitans

Haase, Cristin B. (2013) The Production of TNF-a by RAW 264.7 Macrophage Cells as a Result of Exposure to Aggregatibacter Actinomycetemcomitans. [Abstract]

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Abstract

The goal of this research was to link a common periodontal pathogen to the chronic inflammation of type 2 diabetes through the production of TNF-a (Tumor Necrosis Factor – alpha) by inflammatory cells. TNF-a has been shown to interrupt the insulin pathway by deactivating tyrosine kinase phosphorylation required for glucose uptake. Aggregatibacter actinomycetemcomitans (A.A.) is a gram-negative bacterium found in the oral cavity in numbers that directly correlate to the severity of periodontitis. Heat-killed A.A. were suspended in media and RAW 264.7 macrophage cells were exposed to a negative control of no treatment, a positive control of lipopolysaccharide (LPS) known to induce TNF-a production, or a concentration of 5.9x105 of heat killed Aggregatibacter actinomycetemcomitans. The amount of TNF-a produced by the macrophages was measured using a TNF-a Quantikine Elisa from R&D Systems. The macrophage cells that received treatment of heat killed A.A. and LPS produced significantly larger amounts of TNF-a (40 pg/mL and 25 pg/mL, respectively) compared to the negative control of no treatment (3 pg/mL). These results indicate that periodontal pathogens, such as Aggregatibacter actinomycetemcomitans, through chronic inflammation, have the potential to induce insulin resistance that may lead to type 2 diabetes.

Item Type: Abstract
Created by Student or Faculty: Student
Additional Information: 8th Annual Natural & Behavioral Sciences Undergraduate Research Symposium Program
Uncontrolled Keywords: Tumor Necrosis Factor – alpha, Aggregatibacter actinomycetemcomitans, type 2 diabetes, periodontitis, macrophage cells, pathogens
Subjects: School of Natural and Behavioral Sciences > Biology
School of Natural and Behavioral Sciences > Chemistry
NBS Symposium
Interdisciplinary > Pre-Health
School of Natural and Behavioral Sciences > Public Health
Depositing User: Alejandro Marquez
Date Deposited: 09 May 2013 16:33
Last Modified: 09 May 2013 16:33
URI: http://eprints.fortlewis.edu/id/eprint/234


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