The Effect of Insulin and Estrogen On IRS-1 Phosphorylation in Undifferentiated Muscle Cells.

Shepard, Irelyn and Timmers, Kristjana and Byrd , Shere and Hutchins , Ginny (2012) The Effect of Insulin and Estrogen On IRS-1 Phosphorylation in Undifferentiated Muscle Cells. [Abstract]

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Abstract

Due to close interactions in intracellular signaling, breast cancer and glucose regulation by insulin are linked. In our research we focused on the effects of insulin and estrogen on IRS-1 phosphorylation in L6 myoblasts. Because downstream signaling interactions may be additive, our hypothesis was that there would be an increase in serine 307 phosphorylation in the IRS-1 when undifferentiated cells were treated with both insulin and estrogen. Our research included a series of methods: 1) cell culture, 2) cell treatments of ethanol (control), insulin, estrogen or insulin and estrogen, 3) Immunoprecipitation, 4) PAGE gel, 5) Western blot, and 6) Band analysis. Our results indicated that when Ser 307 phosphorylation was normalized to the total IRS-1 in each group, there were no significant differences between the control and treatment groups, or among any of the treatment groups. The larger implications of our results are that when estrogen and insulin are combined in treatment, there is no effect on Ser 307 phosphorylation.

Item Type: Abstract
Created by Student or Faculty: Student
Additional Information: 7th Annual Natural & Behavioral Sciences Undergraduate Research Symposium Program
Uncontrolled Keywords: Intracellular signaling, Breast cancer, Glucose regulation, Insulin, Estrogen, IRS-1 phosphorylation in L6 myoblasts, Serine 307 phosphorylation, Cell culture, Cell treatments of ethanol (control), Immunoprecipitation, PAGE gel, Western blot, Band analysis, Muscle cells
Subjects: School of Natural and Behavioral Sciences > Biology
NBS Symposium
Depositing User: Alejandro Marquez
Date Deposited: 26 Jun 2013 14:13
Last Modified: 26 Jun 2013 14:13
URI: http://eprints.fortlewis.edu/id/eprint/277


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