Investigating Whether Htz.1 is Located at The ADE2-tel Gene in S. Cerevisiae Strain UCC1371

DeBauche, Spencer and Ziemke, Michael K. (2011) Investigating Whether Htz.1 is Located at The ADE2-tel Gene in S. Cerevisiae Strain UCC1371. [Abstract]

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Abstract

In S. cerevisiae, the Htz.1 variant commonly substitutes the H2A histone in heterochromatin regions. Htz.1 is known to bind to the basal promoter region (TATA box) of the gene. Our study is looking at the possible association of Htz.1 with an artificially placed telomeric gene, ADE2 within the S. cerevisiae mutant UCC1371. We were able to gain insight on this plausible interaction between the two, by utilizing chromatin immunoprecipitation (ChIP) and qPCR technology. From our qPCR results we can speculate that Htz.1 may be located at the ADE2 gene.

Item Type: Abstract
Created by Student or Faculty: Student
Additional Information: 6th Annual Natural & Behavioral Sciences Undergraduate Research Symposium Program
Uncontrolled Keywords: Htz.1, ADE2-tel Gene, S. Cerevisiae Strain UCC1371, Biochemistry, Chromatin immunoprecipitation (ChIP), qPCR technology
Subjects: School of Natural and Behavioral Sciences > Biology
School of Natural and Behavioral Sciences > Chemistry
NBS Symposium
Depositing User: Alejandro Marquez
Date Deposited: 22 May 2013 09:18
Last Modified: 22 May 2013 09:18
URI: http://eprints.fortlewis.edu/id/eprint/331


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