Determining Potential Glacial Refugia FOR Ostrya knowlyonii across the Southwester US as Inferred Through Chloroplast Haplotype Analysis

Paces, Margo and Berg, Kelsey and Wilmer, Chelsea and McGuire, Matthew (2016) Determining Potential Glacial Refugia FOR Ostrya knowlyonii across the Southwester US as Inferred Through Chloroplast Haplotype Analysis. [Abstract]

Full text not available from this repository. (Request a copy)

Abstract

Knowlton Hop-Hornbeam (Ostrya knowltonii) is a small understory tree that is a member of the ancient Arcto-Tertiary flora extending across the Northern Hemisphere. According to the fossil record Ostrya once had an almost continues distribution in North America. Today Ostrya knowltonii exhibits a highly disjunct distribution across the southwestern United States and northern Mexico likely as a result of past climatic conditions. We investigated patterns of genetic relatedness and variation among populations across the region to uncover phylogeographic patterns relevant to past distribution and migration of the species. Ten populations of Ostrya were sampled with DNA from five individuals from each population extracted. Two non-coding chloroplast DNA regions, psbA-trnH and ndhF-rpl32 were amplified using the polymerase chain reaction. The sequences from the two chloroplast DNA plastid regions were then analyzed and reduced to haplotypes projected in geographical space. Four haplotypes were identified in psbA-trnH and five haplotypes in ndhF-rpl32. Similar geographic patterns were observed for both regions. The eastern portion of the range in SE NM and W TX shared identical haplotype structure. Similar haplotypes were also seen in the Colorado River drainage and adjacent areas of N AZ. Slight haplotype differentiation was noted into SE UT, central AZ, and Chihuahua in NW Mexico. The diversity of haplotypes is principally fixed in individual populations pointing to long-term isolation. Limited haplotype sharing in the region of highest population abundance points to persistence of ancestral haplotypes within individual refugial regions.

Item Type: Abstract
Created by Student or Faculty: Student
Uncontrolled Keywords: Phylogeography, Ostrya knowltonii, haplotype
Subjects: Undergrad Research Symposium > Biology
FLC Undergraduate Research & Creative Activities Grant-Funded
Undergrad Research Symposium
Depositing User: Kelsey Berg
Date Deposited: 11 Apr 2016 16:25
Last Modified: 11 Apr 2016 16:25
URI: http://fortworks.fortlewis.edu/id/eprint/769


© FortWorks - powered by EPrints 3 - sponsored and maintained by the John F. Reed Library at Fort Lewis College